"Simplifying Sample Preparation"
R&D Magazine
September, 2005
Preparation of microorganisms for qualitative and quantitative analysis typically consists of multi-step protocols and laborious, time-consuming manipulations such as freezing, boiling, and pulverization. In an effort to streamline this process, researchers at Atmospheric Glow Technologies, Inc., Knoxville, Tenn., developed the PlasmaGen APR-510-S, a benchtop research instrument that generates atmospheric plasma to rapidly liberate nucleic acid from microorganisms in a one-step, reagent-free process. Amplifiable DNA is available in two minutes or less, even from a mixed population with this tool. Moreover, the PlasmaGen prepares DNA for ananlysis on dry sample matrices, providing an alternative to current methods that rely strictly on wet chemistry. By avoiding wet chemistry, multiple wash and recovery steps are avoided, greatly decreasing the risk of losing samples that may be present in low numbers. By manipulating samples only once, there is less risk for cross-contamination and reduced exposure of the technician to potential pathogens.